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ผลงานตีพิมพ์ในวารสารวิชาการHaematology, cytochemical and ultrastructural characteristics of blood cells in leopard (Panthera pardus) ผู้แต่ง: Dr.Chaleow Salakij, Professor , Mr.Jarernsak Salakij, Associate Professor ,  นวลอนงค์ นาคคง, Mr.Kreangsak Prihirunkit, Associate Professor, สุเมษ กมลนรนารถ, สุนทรี อภิบาล, วารสาร:  
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Morphology and Cytochemistry of Blood Cells from Asian Wild Dog (Cuon alpinus)) ผู้เขียน:ดร.เฉลียว ศาลากิจ, ศาสตราจารย์, นายเจริญศักดิ์ ศาลากิจ, รองศาสตราจารย์, นางสาวจุฑามาส รัตนคุณูประการ , Narit Tengchaisri, Wanchai Tunwattana, สุนทรี อภิบาล สื่อสิ่งพิมพ์:pdf AbstractBlood cells from direct blood smears of four adult Asian wild dogs (Cuon alpinus) were examined and measured after stainning with modified Wrights Giemsa stain and cytochemical stains, including Sudan black B (SBB), ?-naphthyl acetate esterase (ANAE), and ?-glucuronidase (?-glu). Red blood cells show uniform in sized, biconcave and prominent central pallor with 6.0 ?m mean diameter. Reticulocytes and nucleated red blood cells stained positive for ANAE and ?-glu. Neutrophils have tight constricted and multilobulated nucleus which mostly occupied the cells. Neutrophils stain strongly positive with SBB, negative for ANAE and weak positive for ?-glu. Eosinophils contain numerous round red refractile granules with some vacuoles. Eosinophils stain moderately positive for SBB and ?-glu but negative for ANAE. Basophils have variable numbers of intensely basophilic granules which do not obscure the very long lobulated nucleus. Basophils stain moderately positive for SBB but strongly positive for ANAE and ?-glu. Most lymphocytes are small and medium size (65:68). Lymphocytes are negative for SBB but have 3 patterns of reactivity for ANAE and ?-glu, including negative, focal dot staining and fine granular staining. Monocytes stain moderately positive for SBB and moderately to strongly positive for ANAE and ?-glu. Platelets are negative for SBB and stain moderately to strongly positive for ANAE and ?-glu. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Hematology, Cytochemistry and Ultrastructure of Blood Cells from Asian Elephant (Elephas maximus)) ผู้เขียน:นายเจริญศักดิ์ ศาลากิจ, รองศาสตราจารย์, ดร.เฉลียว ศาลากิจ, ศาสตราจารย์, นางสาวนวลอนงค์ นาคคง, สุนทรี อภิบาล, Piyawan Suthunmapinuntra, Jutamat Rattanakukuprakarn, Gavil Nunklang, Manoch Yindee สื่อสิ่งพิมพ์:pdf AbstractBlood cells from fourteen (5 males and 9 females) adult Asian elephants were examined and cytochemical stained with Sudan Black B (SBB), peroxidase, periodic acid Schiff’s reaction (PAS), anaphthyl acetate esterase (ANAE) and b-glucuronidase (b-glu). The complete blood counts were performed using automate cell counter. There were no significant differences nearly all hematological values between the male and female elephants except the leukocyte count and fibrinogen concentration which were higher and lower, respectively, in the males than the females. Neutrophils had poorly segmented neclei and many well-differentiated granules. Neutrophils stained strongly positive to SBB, faintly stained with PAS, focal dot stained to ANAE and b-glu. Eosinophils contained 2-3 lobe nuclei, numerous small round red refractive granules with some vacuoles. Eosinophils stained moderately positive to SBB and strongly positive to ANAE but negative to b-glu. Basophils had variable number of intensely basophilic granules which did not obscure the lobed nuclei. Basophils were negative for SBB but moderately positive to ANAE and b-glu. Monocytes stained moderately positive to SBB and moderately to strongly positive to ANAE and b-glu. The bilobed cells stained moderately positive for SBB and strongly positive for ANAE and b-glu which were similar to monocytes. Ultrastructurally, they contained a large number of mitochondria similar to those of monocytes except the shape of the nuclei. The number of bilobed cells exceeded the number of the other leukocytes. Scanning electron microscopy revealed surfaces of all blood cells. Transmission electron microscopy revealed organelles within erythrocytes, platelets and all leukocytes especially bilobed cells. |
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